1TQS
Golgi alpha-Mannosidase II In Complex With Salacinol
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-03-24 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9504 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 68.824, 109.631, 138.852 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.330 - 1.300 |
| R-factor | 0.163 |
| Rwork | 0.163 |
| R-free | 0.18200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hty |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.900 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.320 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.110 | 0.361 |
| Number of reflections | 256327 | |
| <I/σ(I)> | 14.5 | 5.4 |
| Completeness [%] | 99.2 | 95.6 |
| Redundancy | 6.75 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 295 | PEG 6000, MPD, TRIS, NaCl, pH 7.00, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
