1TQO
Cryogenic Crystal Structure of Staphylococcal Nuclease Variant truncated Delta+PHS I92E
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 95 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-05-20 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 33.820, 59.430, 73.420 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.130 - 2.000 |
| R-factor | 0.217 |
| Rwork | 0.217 |
| R-free | 0.24900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1snc |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.300 | 2.130 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.051 | 0.096 |
| Number of reflections | 10439 | |
| <I/σ(I)> | 15.81 | 4.81 |
| Completeness [%] | 99.6 | 99.9 |
| Redundancy | 6 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 300 | MPD, potassium phosphate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 27K |
