1TQ8

Crystal Structure of protein Rv1636 from Mycobacterium tuberculosis H37Rv

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X9A
Synchrotron site	NSLS
Beamline	X9A
Temperature [K]	100
Detector technology	CCD
Collection date	2004-04-30
Detector	MARRESEARCH
Wavelength(s)	0.98
Spacegroup name	P 21 21 2
Unit cell lengths	72.829, 121.042, 119.751
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.940 - 2.400
R-factor	0.221
Rwork	0.221
R-free	0.26200
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	1.100
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.490
High resolution limit [Å]	2.400	2.400
Number of reflections	79424
<I/σ(I)>	19.54
Completeness [%]	99.3	93.4
Redundancy	3.74	2.39

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	291	1.6M NaCl, 0.1M NaAcetate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K
1	VAPOR DIFFUSION, HANGING DROP	5	291	1.6M NaCl, 0.1M NaAcetate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K
1	VAPOR DIFFUSION, HANGING DROP	5	291	1.6M NaCl, 0.1M NaAcetate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K