1TQ3

Higher resolution crystal structure of the third PDZ domain of post synaptic PSD-95 protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-C
Synchrotron site	APS
Beamline	14-BM-C
Temperature [K]	100
Detector technology	CCD
Collection date	2003-07-23
Detector	MARRESEARCH
Wavelength(s)	1.0
Spacegroup name	P 41 3 2
Unit cell lengths	89.262, 89.262, 89.262
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.960 - 1.890
R-factor	0.23812
Rwork	0.235
R-free	0.29575
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.036
RMSD bond angle	2.554
Data reduction software	XDS
Data scaling software	d*TREK
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall
Low resolution limit [Å]	28.230
High resolution limit [Å]	1.890
Number of reflections	10234

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.9	295	1.0 M sodium citrate, 0.1 M HEPES, pH 6.9, VAPOR DIFFUSION, HANGING DROP, temperature 295K