1TPU

S96P CHANGE IS A SECOND-SITE SUPPRESSOR FOR H95N SLUGGISH MUTANT TRIOSEPHOSPHATE ISOMERASE

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	136.300, 74.100, 57.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 * - 1.950 *
R-factor	0.176
Rwork	0.176
RMSD bond length	0.014
RMSD bond angle	2.670
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	13.000 *
High resolution limit [Å]	1.950 *
Rmerge	0.077 *
Total number of observations	114538 *
Number of reflections	39362 *
Completeness [%]	92.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8.5 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	reservoir	Tris-HCl	100 (mM)
3	1	reservoir	lithium sulfate	200 (mM)
4	1	reservoir	PEG8000	12.5-21 (%)