1TPL

THE THREE-DIMENSIONAL STRUCTURE OF TYROSINE PHENOL-LYASE

Experimental procedure

Spacegroup name	P 21 21 2
Unit cell lengths	76.020, 138.270, 93.540
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.300
R-factor	0.1622 *
RMSD bond length	0.012
RMSD bond angle	0.038 *
Refinement software	PROLSQ

Data quality characteristics

	Overall
Low resolution limit [Å]	15.000 *
High resolution limit [Å]	2.300 *
Rmerge	0.058 *
Redundancy	4.1 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	7 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	25-30 (mg/ml)
2	1	drop	potasssium phosphate	50 (mM)
3	1	drop	ammonium sulfate	20 (%sat)
4	1	drop	dithiothreitol	0.2 (mM)
5	1	reservoir	ammonium sulfate	30 (%sat)