1TP8
CRYSTAL STRUCTURE OF A GALACTOSE SPECIFIC LECTIN FROM ARTOCARPUS HIRSUTA IN COMPLEX WITH METHYL-a-D-GALACTOSE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 295 |
Detector technology | IMAGE PLATE |
Detector | MARRESEARCH |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 89.900, 121.900, 131.600 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 3.000 |
Rwork | 0.193 |
R-free | 0.23740 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1jac |
RMSD bond length | 0.008 |
RMSD bond angle | 2.200 |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.730 | 3.500 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.097 | 0.202 |
Number of reflections | 28782 | |
<I/σ(I)> | 19.3 | 7.22 |
Completeness [%] | 98.9 | 99.5 |
Redundancy | 4.55 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 295 | 0.2 M PO4 BUFFER, 35-40% AMMONIUM SULPHATE,METHYL ALPHA GALACTOSE, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |