1TP6
1.5 A Crystal Structure of a NTF-2 Like Protein of Unknown Function PA1314 from Pseudomonas aeruginosa
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-05-29 |
| Detector | SBC-3 |
| Wavelength(s) | 0.9795,0.9797,0.94656 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 96.802, 36.309, 29.570 |
| Unit cell angles | 90.00, 100.71, 90.00 |
Refinement procedure
| Resolution | 29.060 - 1.500 |
| R-factor | 0.195 |
| Rwork | 0.185 |
| R-free | 0.22600 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.100 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.043 | 0.252 |
| Number of reflections | 30861 | |
| <I/σ(I)> | 35.07 | 3.8 |
| Completeness [%] | 97.5 | 80.7 |
| Redundancy | 4.4 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 298 | 0.1 M Sodium Acetate, 0.2 M Sodium Citrate, 4% Glycerol, 25% PEG 3350, pH 4.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
