1TOH
TYROSINE HYDROXYLASE CATALYTIC AND TETRAMERIZATION DOMAINS FROM RAT
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-06 |
| Detector | MARRESEARCH |
| Spacegroup name | F 2 2 2 |
| Unit cell lengths | 189.323, 148.335, 57.975 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.000 - 2.300 |
| R-factor | 0.2109 |
| Rwork | 0.211 |
| R-free | 0.26800 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.019 |
| RMSD bond angle | 22.800 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS (0.3) |
| Refinement software | CNS (0.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.042 | 0.187 |
| Total number of observations | 126857 * | |
| Number of reflections | 18068 | |
| <I/σ(I)> | 21.3 | 5 |
| Completeness [%] | 98.0 | 92 |
| Redundancy | 7 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Microdialysis * | 7.9 | 4 * | 1.2 M AMMONIUM SULFATE 3.2% PEG 200 5% GLYCEROL 2 MM DTT 80 MM TRIS, PH 7.9 CRYSTALS GROWN AT 4 DEGREES C, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 8 (mg/ml) | |
| 10 | 1 | 2 | dithiothreitol | 2 (mM) | |
| 2 | 1 | 1 | ammonium sulfate | 120 (mM) | |
| 3 | 1 | 1 | Tris-HCl | 100 (mM) | |
| 4 | 1 | 1 | glycerol | 5 (%) | |
| 5 | 1 | 1 | dithiothreitol | 2 (mM) | |
| 6 | 1 | 2 | ammonium sulfate | 1.15 (M) | |
| 7 | 1 | 2 | PEG200 | 3.2 (%) | |
| 8 | 1 | 2 | glycerol | 5 (%) | |
| 9 | 1 | 2 | Tris-HCl | 80 (mM) |
