1TOA
PERIPLASMIC ZINC BINDING PROTEIN TROA FROM TREPONEMA PALLIDUM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 1998-10-15 |
| Detector | ADSC |
| Spacegroup name | P 61 |
| Unit cell lengths | 126.200, 126.200, 74.480 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.800 |
| Rwork | 0.176 |
| R-free | 0.20300 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.008 |
| RMSD bond angle | 23.100 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.840 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.043 * | 0.280 * |
| Number of reflections | 62551 | |
| <I/σ(I)> | 38.9 | 4.25 |
| Completeness [%] | 99.9 | 99.7 |
| Redundancy | 3.66 | 3.37 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 | 20 * | pH 7.0 |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | HEPES | ||
| 2 | 1 | 1 | LICL | ||
| 3 | 1 | 1 | PEG 4000 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | Na-HEPES | 0.1 (M) | |
| 2 | 1 | reservoir | 1.0 (M) | ||
| 3 | 1 | reservoir | PEG4000 | 30 (%) | or PEG6000 |
