1TND

THE 2.2 ANGSTROMS CRYSTAL STRUCTURE OF TRANSDUCIN-ALPHA COMPLEXED WITH GTP GAMMA S

Experimental procedure

Spacegroup name	P 1 21 1
Unit cell lengths	74.500, 108.300, 79.000
Unit cell angles	90.00, 112.30, 90.00

Refinement procedure

Resolution	6.000 - 2.200
R-factor	0.178 *
Rwork	0.190
R-free	0.30400 *
RMSD bond length	0.011
RMSD bond angle	1.800
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	12.600 *
High resolution limit [Å]	2.800 *
Rmerge	0.039 *
Number of reflections	53289 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6 *	-12.5 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20-25 (mg/ml)
10	1	reservoir	beta-mercaptoethanol	25 (mM)
11	1	reservoir	glycerol	30 (%(v/v))
2	1	drop	sodium cacodylate	166 (mM)
3	1	drop		223 (mM)
4	1	drop	beta-mercaptoethanol	25 (mM)
5	1	drop	glycerol	20 (%(v/v))
6	1	drop	PEG8000	3.33 (%(w/v))
7	1	reservoir	PEG8000	5 (%(w/v))
8	1	reservoir	sodium cacodylate	250 (mM)
9	1	reservoir		350 (mM)