1TM2

Crystal Structure of the apo form of the Salmonella typhimurium AI-2 receptor LsrB

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200H
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	37.984, 73.979, 116.244
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.130 - 1.900
R-factor	0.193
Rwork	0.191
R-free	0.22700
Structure solution method	MAD
RMSD bond length	0.005
RMSD bond angle	1.200
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.130	2.020
High resolution limit [Å]	1.900	1.900
Number of reflections	25812
Completeness [%]	97.0	93.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	298	PEG 4000, NaCl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 25K
1	VAPOR DIFFUSION, HANGING DROP	8.5	298	PEG 4000, NaCl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 25K
1	VAPOR DIFFUSION, HANGING DROP	8.5	298	PEG 4000, NaCl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 25K