1TM0
Crystal Structure of the putative proline racemase from Brucella melitensis, Northeast Structural Genomics Target LR31
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-04-04 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97904 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 138.027, 77.363, 77.416 |
| Unit cell angles | 90.00, 124.11, 90.00 |
Refinement procedure
| Resolution | 28.570 - 2.800 |
| R-factor | 0.236 |
| Rwork | 0.236 |
| R-free | 0.31200 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SnB |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.570 | 2.980 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.078 | 0.181 |
| Number of reflections | 26270 | |
| <I/σ(I)> | 13.5 | 5.1 |
| Completeness [%] | 84.3 | 98.2 |
| Redundancy | 3.4 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 20% PEG 3350, pH 7, 200mM potassium thiocyanate, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
