1TLW
Tsx structure complexed with thymidine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Wavelength(s) | 0.9790 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 149.653, 149.653, 119.939 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.340 - 3.100 |
| R-factor | 0.297 |
| Rwork | 0.269 |
| R-free | 0.29700 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 3.100 |
| Number of reflections | 27650 |
| Completeness [%] | 97.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | soaking the crystal with 10 mM thymidine | 4.3 | 286 | PEG550 27-32%, sodium acetate 50 mM, glycerol 10%, pH 4.3, soaking the crystal with 10 mM thymidine, temperature 286K |
