1TLQ

Crystal structure of protein ypjQ from Bacillus subtilis, Pfam DUF64

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X9A
Synchrotron site	NSLS
Beamline	X9A
Temperature [K]	100
Detector technology	CCD
Collection date	2004-04-27
Detector	MARRESEARCH
Wavelength(s)	0.98
Spacegroup name	P 64 2 2
Unit cell lengths	118.112, 118.112, 65.792
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.400
R-factor	0.23534
Rwork	0.232
R-free	0.29466
Structure solution method	SAD
Starting model (for MR)	Experimental electron density from Selenium SAD experiment.
RMSD bond length	0.046
RMSD bond angle	3.232
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.490
High resolution limit [Å]	2.400	2.400
Number of reflections	20050
<I/σ(I)>	31.75	4.45
Completeness [%]	99.8	99.9
Redundancy	11.15

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.75	291	26% Peg 4k, 0.1M Tris, 0.8M Lithium Chloride, pH 8.75, VAPOR DIFFUSION, HANGING DROP, temperature 291K