1TLG
STRUCTURE OF A TUNICATE C-TYPE LECTIN COMPLEXED WITH D-GALACTOSE
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | ELLIOTT GX-13 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-08 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 54.180, 66.450, 85.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.000 - 2.200 |
| R-factor | 0.211 * |
| Rwork | 0.211 |
| R-free | 0.26900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1byf |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.025 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 16.400 | 2.240 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.056 | 0.171 |
| Total number of observations | 108587 * | |
| Number of reflections | 16111 | |
| <I/σ(I)> | 11.6 | 4.4 |
| Completeness [%] | 99.1 | 83.8 |
| Redundancy | 6.7 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.2 * | 17 * | pH 6.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 0.4 (mM) | |
| 2 | 1 | drop | 12.5 (mM) | ||
| 3 | 1 | drop | D-galactose | 2.5 (mM) | |
| 4 | 1 | drop | Tris | 10 (mM) | |
| 5 | 1 | drop | 25 (mM) | ||
| 6 | 1 | reservoir | PEG8000 | 7 (%(w/v)) | |
| 7 | 1 | reservoir | 0.2 (M) | ||
| 8 | 1 | reservoir | sodium cacodylate | 0.1 (M) |
