1TLF

UNPRECEDENTED QUATERNARY STRUCTURE OF E. COLI LAC REPRESSOR CORE TETRAMER: IMPLICATIONS FOR DNA LOOPING

Experimental procedure

Spacegroup name	P 1 21 1
Unit cell lengths	90.160, 64.730, 117.940
Unit cell angles	90.00, 91.75, 90.00

Refinement procedure

Resolution	8.000 * - 2.600
R-factor	0.222
Rwork	0.222
RMSD bond length	0.012
RMSD bond angle	1.800
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	8.000
High resolution limit [Å]	2.600
Number of reflections	37549
Completeness [%]	97.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	NaMES	10 (mM)
2	1	1	IPTG	0.1 (mM)
3	1	1		0.02 (%(w/v))
4	1	1	ethylmercury phosphate	17 (mg/ml)
5	1	2	NaMES	100 (mM)
6	1	2	PEG6000	4 (%(w/v))
7	1	2	IPTG	0.1 (mM)
8	1	2		0.02 (%(w/v))
9	1	1	EDTA	0.010 (mM)