1TLB
Yeast coproporphyrinogen oxidase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-01-29 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 232.773, 65.238, 166.071 |
| Unit cell angles | 90.00, 107.85, 90.00 |
Refinement procedure
| Resolution | 158.110 - 2.400 |
| R-factor | 0.21189 |
| Rwork | 0.208 |
| R-free | 0.28239 |
| Structure solution method | Chromium sulfur SAD |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.832 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.550 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.077 | 0.309 |
| Number of reflections | 83203 | |
| <I/σ(I)> | 15 | 2.5 |
| Completeness [%] | 93.9 | 90.7 |
| Redundancy | 5 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 294 | 2.2 M Ammonium Sulfate, 0.1 M Tris buffer, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
