1TKS

Crystal structure of 3,4-Dihydroxy-2-butanone 4-phosphate Synthase of Candida albicans

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2003-07-03
Detector	MARRESEARCH
Wavelength(s)	1.542
Spacegroup name	P 1
Unit cell lengths	40.439, 48.134, 59.804
Unit cell angles	66.23, 71.98, 89.86

Refinement procedure

Resolution	20.000 - 1.600
Rwork	0.192
R-free	0.21900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1g57
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.660
High resolution limit [Å]	1.600	1.600
Rmerge	0.059	0.221
Number of reflections	43264
<I/σ(I)>	27.7
Completeness [%]	84.4	81.9
Redundancy	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	293	Na-Citrat, PEG8000, pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K