1TKL
Yeast Oxygen-Dependent Coproporphyrinogen Oxidase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-02-16 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.10 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 86.822, 86.822, 207.835 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 79.060 - 2.000 |
| R-factor | 0.2101 |
| Rwork | 0.208 |
| R-free | 0.25828 |
| Structure solution method | Chromium sulfur SAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.507 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.057 | 0.583 |
| Number of reflections | 51064 | |
| <I/σ(I)> | 19 | 3.2 |
| Completeness [%] | 98.4 | 97.5 |
| Redundancy | 9 | 9.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 18% PEG 8000, 0.1 M HEPES, 2% isopropanol, 0.2 M sodium acetate, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
