1TJE

Crystal structure of the editing domain of threonyl-tRNA synthetase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Detector technology	CCD
Collection date	2003-08-04
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9763
Spacegroup name	P 21 21 21
Unit cell lengths	43.566, 45.243, 116.602
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.800 - 1.500
R-factor	0.203
Rwork	0.202
R-free	0.22400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1qf6
RMSD bond length	0.006
RMSD bond angle	1.300
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.800	1.550
High resolution limit [Å]	1.500	1.500
Rmerge	0.077	0.382
Number of reflections	32601
Completeness [%]	85.8	79
Redundancy	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	281	PEG 4000, Tris.cl, magnesium chloride, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 281K