1TJD
The crystal structure of the reduced disulphide bond isomerase, DsbC, from Escherichia coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-10-19 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.906 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 41.897, 145.799, 73.582 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 16.740 - 2.500 |
| R-factor | 0.2043 |
| Rwork | 0.203 |
| R-free | 0.26964 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1eej |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.271 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 17.000 | 2.540 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.081 | 0.386 |
| Number of reflections | 7953 | |
| <I/σ(I)> | 14 | 2.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 11 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.4 | 293 | 0.2 M LiSO4, 0.1 M Tris pH 8.4 and 20% w/v PEG 4000, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
