1TJC
Crystal structure of peptide-substrate-binding domain of human type I collagen prolyl 4-hydroxylase
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-01-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9814, 0.9811, 0.9745 |
| Spacegroup name | P 32 |
| Unit cell lengths | 55.980, 55.980, 105.430 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.580 - 2.300 |
| R-factor | 0.2257 |
| Rwork | 0.224 |
| R-free | 0.25872 |
| Structure solution method | MAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.677 |
| Data scaling software | XDS |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.400 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.038 | 0.258 |
| Number of reflections | 16195 | |
| <I/σ(I)> | 15.66 | 3.7 |
| Completeness [%] | 98.7 | 99.2 |
| Redundancy | 3.16 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.4 | 277 | ammonium phosphate, pH 8.4, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
