1TJB
Crystal Structure of a High Affinity Lanthanide-Binding Peptide (LBT)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-07-26 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 52.160, 22.270, 30.030 |
| Unit cell angles | 90.00, 103.89, 90.00 |
Refinement procedure
| Resolution | 17.180 - 2.000 |
| R-factor | 0.1647 |
| Rwork | 0.161 |
| R-free | 0.19530 |
| Structure solution method | SAD PHASING |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.107 | 0.171 |
| Number of reflections | 2315 | |
| <I/σ(I)> | 15.2 | 7.8 |
| Completeness [%] | 99.1 | 100 |
| Redundancy | 3.6 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | t-Butanol, Terbium Chloride, sodium chloride, tris, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
