1TIB

CONFORMATIONAL LABILITY OF LIPASES OBSERVED IN THE ABSENCE OF AN OIL-WATER INTERFACE: CRYSTALLOGRAPHIC STUDIES OF ENZYMES FROM THE FUNGI HUMICOLA LANUGINOSA AND RHIZOPUS DELEMAR

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	103.165, 51.994, 45.733
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	7.500 - 1.850 *
R-factor	0.167 *
RMSD bond length	0.016 *
RMSD bond angle	2.900 *
Refinement software	PROLSQ

Data quality characteristics

	Overall
High resolution limit [Å]	1.850 *
Rmerge	0.047 *
Completeness [%]	100.0 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	1.2 (%)
2	1	reservoir	PEG6000	4 (%)
3	1	reservoir	sodium acetate	100 (mM)
4	1	reservoir		1 (mM)	can be replaced by NaAuCl2
5	1	reservoir	spermidine	3 (mM)