1TIA
AN UNUSUAL BURIED POLAR CLUSTER IN A FAMILY OF FUNGAL LIPASES
Experimental procedure
| Spacegroup name | P 1 |
| Unit cell lengths | 45.570, 47.100, 33.500 |
| Unit cell angles | 79.05, 112.14, 70.28 |
Refinement procedure
| Resolution | 7.500 - 2.100 |
| R-factor | 0.19 |
| Rwork | 0.190 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 0.061 * |
| Phasing software | X-PLOR |
| Refinement software | PROLSQ |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 1.980 * |
| Rmerge | 0.051 * |
| Completeness [%] | 79.9 * |
| Redundancy | 1.97 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 1 (%) | |
| 2 | 1 | drop | DTT | 2 (mM) | |
| 3 | 1 | reservoir | PEG2000 | 10 (%) | |
| 4 | 1 | reservoir | sodium acetate | 100 (mM) | |
| 5 | 1 | reservoir | DTT | 2 (mM) |
