1TGV

Structure of E. coli Uridine Phosphorylase complexed with 5-Fluorouridine and sulfate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	100
Detector technology	CCD
Collection date	2003-08-10
Detector	ADSC QUANTUM 4
Spacegroup name	H 3
Unit cell lengths	149.972, 149.972, 49.566
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.200
Rwork	0.175
R-free	0.22400
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.300
Data reduction software	MOSFLM
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.200
Number of reflections	19412
<I/σ(I)>	14.4
Completeness [%]	91.9
Redundancy	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.2	298	PEG 4000, MES, Glycerol, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K