1TGU
The crystal structure of bovine liver catalase without NADPH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12B |
| Synchrotron site | NSLS |
| Beamline | X12B |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 85.530, 139.670, 225.140 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.800 |
| R-factor | 0.2 |
| Rwork | 0.200 |
| R-free | 0.24900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 3.000 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.800 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.114 | 0.436 |
| Number of reflections | 64055 | |
| Completeness [%] | 68.3 | |
| Redundancy | 2.8 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 273 | Tris Hcl buffer, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 273K |
