1TGL

A SERINE PROTEASE TRIAD FORMS THE CATALYTIC CENTRE OF A TRIACYLGLYCEROL LIPASE

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	71.600, 75.000, 55.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	7.500 - 1.900
R-factor	0.138
RMSD bond length	0.029 *
Refinement software	PROLSQ

Data quality characteristics

	Overall
High resolution limit [Å]	1.900 *
Rmerge	0.081 *
Number of reflections	21165 *
Completeness [%]	87.8 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8.05 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	Tris-HCl	20 (mM)
2	1	drop	protein	15-16 (mg/ml)
3	1	reservoir	phosphate	55-75 (%(v/v)sat)