1TGG
RH3 DESIGNED RIGHT-HANDED COILED COIL TRIMER
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL1-5 |
| Synchrotron site | SSRL |
| Beamline | BL1-5 |
| Detector technology | AREA DETECTOR |
| Wavelength(s) | 1.4862, 1.4851, 1.3931 |
| Spacegroup name | P 32 |
| Unit cell lengths | 25.420, 25.420, 141.340 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 12.000 - 2.000 |
| R-factor | 0.236 |
| Rwork | 0.233 |
| R-free | 0.26900 |
| Structure solution method | MAD |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MLPHARE |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 12.000 |
| High resolution limit [Å] | 2.000 |
| Number of reflections | 11956 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 100mM Tris pH 8.5, 0.3M NaBr, 15mM NiCl2, 15% methyl pentanediol, 15% polyethylene glycol 4000, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
