1TG7
Native structure of beta-galactosidase from Penicillium sp.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE D03B-MX1 |
| Synchrotron site | LNLS |
| Beamline | D03B-MX1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-01-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 43 |
| Unit cell lengths | 110.960, 110.960, 161.050 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 22.300 - 1.900 |
| Rwork | 0.166 |
| R-free | 0.18500 |
| Structure solution method | AB INITIO PHASING |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.500 |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.300 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.105 | 0.400 |
| Number of reflections | 152744 | |
| <I/σ(I)> | 13.9 | 3.2 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 291 | 15% PEG 8000, 50 mM sodium phosphate, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
