1TG5
Crystal structures of plant 4-hydroxyphenylpyruvate dioxygenases complexed with DAS645
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-01-01 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 77.464, 83.871, 63.301 |
| Unit cell angles | 90.00, 102.28, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.900 |
| R-factor | 0.191 |
| Rwork | 0.188 |
| R-free | 0.23400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1sqd |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 1.950 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.048 | 0.155 |
| Number of reflections | 30159 | |
| <I/σ(I)> | 12 | 2.5 |
| Completeness [%] | 98.3 | 83.8 |
| Redundancy | 4.4 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.3 | 298 | PEG 4000, isopropanol, Bis-tris, potassium chloride, cobalt chloride, pH 7.3, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
