1TFV
CRYSTAL STRUCTURE OF A BUFFALO SIGNALING GLYCOPROTEIN (SPB-40) SECRETED DURING INVOLUTION
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 295 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-04-15 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5417 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 62.764, 66.559, 107.137 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.900 |
| R-factor | 0.23987 |
| Rwork | 0.237 |
| R-free | 0.28806 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ljy |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.928 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.19) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.950 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Number of reflections | 8070 | |
| <I/σ(I)> | 11 | 2.2 |
| Completeness [%] | 75.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICRODIALYSIS | 8 | 298 | 16% ETHANOL, 25MM TRIS, pH 8.0, MICRODIALYSIS, temperature 298K |
