1TFR

RNASE H FROM BACTERIOPHAGE T4

Experimental procedure

Detector technology	IMAGE PLATE
Collection date	1995-10-18
Detector	RIGAKU
Spacegroup name	P 21 21 21
Unit cell lengths	74.470, 87.580, 54.560
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 2.060
R-factor	0.205 *
Rwork	0.205
R-free	0.29000
RMSD bond length	0.010
RMSD bond angle	0.032
Data reduction software	PROCESS
Refinement software	PROLSQ

Data quality characteristics

	Overall
Low resolution limit [Å]	6.000
High resolution limit [Å]	2.060
Rmerge	0.050
Total number of observations	58561 *
Number of reflections	22706
Completeness [%]	93.6
Redundancy	2.58

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.5 *	21 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (OD280/ml)
2	1	drop		50 (mM)
3	1	reservoir	magnesium acetate	300 (mM)
4	1	reservoir	sodium cacodylate	100 (mM)
5	1	reservoir	PEG8000	18 (%)