1TFM

CRYSTAL STRUCTURE OF A RIBOSOME INACTIVATING PROTEIN IN ITS NATURALLY INHIBITED FORM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Temperature [K]	273
Detector technology	IMAGE PLATE
Collection date	2001-04-13
Detector	MARRESEARCH
Wavelength(s)	0.8453
Spacegroup name	P 65 2 2
Unit cell lengths	109.422, 109.422, 309.798
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.800
R-factor	0.23998
Rwork	0.234
R-free	0.28856
Structure solution method	MIR
Starting model (for MR)	1pc8
RMSD bond length	0.010
RMSD bond angle	1.716
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC (5.1.19)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.850
High resolution limit [Å]	2.800	2.800
Number of reflections	26570
<I/σ(I)>	33.5	2
Completeness [%]	95.8
Redundancy	42.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4	298	NaCl,Ammonium Sulphate, Dioxane, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K