1TFK

Ribonuclease from Escherichia coli complexed with its inhibtor protein

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	95
Detector technology	CCD
Collection date	2003-03-27
Detector	MARRESEARCH
Wavelength(s)	0.97919, 0.97937, 0.98200
Spacegroup name	P 41 21 2
Unit cell lengths	58.503, 58.503, 149.392
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	23.100 - 2.100
R-factor	0.228
Rwork	0.225
R-free	0.25100
Structure solution method	MAD
RMSD bond length	0.005
RMSD bond angle	1.100
Data scaling software	SCALA
Phasing software	SOLVE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	23.100	2.200
High resolution limit [Å]	2.100	2.100
Number of reflections	15793
Completeness [%]	99.4	99.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	277	PEG 8000, DTT, magnesium acetate, MES, MPD, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K