1TF5
Crystal structure of SecA in an open conformation from Bacillus Subtilis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Wavelength(s) | 0.97903 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 149.232, 107.189, 72.054 |
| Unit cell angles | 90.00, 94.96, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.180 |
| R-factor | 0.241 |
| Rwork | 0.241 |
| R-free | 0.27300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1m6n |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.100 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.260 |
| High resolution limit [Å] | 2.180 | 2.180 |
| Number of reflections | 58197 | |
| <I/σ(I)> | 28.37 | |
| Completeness [%] | 98.8 | 97.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 0.1M HEPES, 250mM Magnesium Acetate, 7-12% PEG 8000, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
