1TF2
Crystal structure of SecA:ADP in an open conformation from Bacillus Subtilis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Wavelength(s) | 0.97903 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 149.232, 107.189, 72.054 |
| Unit cell angles | 90.00, 94.96, 90.00 |
Refinement procedure
| Resolution | 44.980 - 2.900 |
| R-factor | 0.228 |
| Rwork | 0.228 |
| R-free | 0.29200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB enrty 1M6N |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Number of reflections | 25014 | |
| Completeness [%] | 99.3 | 94 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 0.1M HEPES, 250mM Magnesium Acetate, 7-12% PEG 8000, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
