1TDR
EXPRESSION, CHARACTERIZATION, AND CRYSTALLOGRAPHIC ANALYSIS OF TELLUROMETHIONYL DIHYDROFOLATE REDUCTASE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X8C |
| Synchrotron site | NSLS |
| Beamline | X8C |
| Detector technology | CCD |
| Collection date | 1992-10-24 |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 61 |
| Unit cell lengths | 93.000, 93.000, 74.400 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 16.000 - 2.500 |
| R-factor | 0.124 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 0.059 |
| Data reduction software | MADNES |
| Refinement software | PROLSQ |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.500 * |
| Rmerge | 0.065 * |
| Total number of observations | 48880 * |
| Number of reflections | 11234 |
| Completeness [%] | 88.0 |
| Redundancy | 4.35 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | 2-propanol | 10 (%) | |
| 2 | 1 | reservoir | PEG4000 | 20 (%) | |
| 3 | 1 | reservoir | sodium HEPES | 0.1 (M) | pH7.5 |
| 4 | 1 | drop | protein | 5 (mg/ml) |
