1TDO
L-amino acid oxidae from Agkistrodon halys in complex with L-phenylalanine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BSRF BEAMLINE 3W1A |
| Synchrotron site | BSRF |
| Beamline | 3W1A |
| Temperature [K] | 300 |
| Detector technology | CCD |
| Wavelength(s) | 1.1 |
| Spacegroup name | I 21 3 |
| Unit cell lengths | 169.410, 169.410, 169.410 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.280 - 3.000 |
| R-factor | 0.179 |
| Rwork | 0.179 |
| R-free | 0.23500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1reo |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | AUTOMAR |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.074 | 0.403 |
| Number of reflections | 133736 | |
| Completeness [%] | 99.6 | |
| Redundancy | 7.06 | 7.42 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 300 | pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 300K |
