1TDN
L-amino acid oxidase from Agkistrodon halys in complex with L-leucine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BSRF BEAMLINE 3W1A |
Synchrotron site | BSRF |
Beamline | 3W1A |
Temperature [K] | 300 |
Detector technology | CCD |
Wavelength(s) | 1.1 |
Spacegroup name | I 21 3 |
Unit cell lengths | 169.530, 169.530, 169.530 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.310 - 2.700 |
R-factor | 0.192 |
Rwork | 0.192 |
R-free | 0.22700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1reo |
RMSD bond length | 0.007 |
RMSD bond angle | 1.300 |
Data reduction software | AUTOMAR |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.060 | 0.388 |
Number of reflections | 169431 | |
Completeness [%] | 100.0 | 100 |
Redundancy | 7.31 | 7.31 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 300 | pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 300K |