1TDK
L-amino acid oxidase from Agkistrodon halys in complex with suicide substrate L-vinylglycine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BSRF BEAMLINE 3W1A |
| Synchrotron site | BSRF |
| Beamline | 3W1A |
| Temperature [K] | 300 |
| Detector technology | CCD |
| Spacegroup name | I 21 3 |
| Unit cell lengths | 169.360, 169.360, 169.360 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.260 - 2.700 |
| R-factor | 0.192 |
| Rwork | 0.192 |
| R-free | 0.22100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1reo |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | AUTOMAR |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.750 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Rmerge | 0.051 | 0.447 |
| Number of reflections | 176710 | |
| <I/σ(I)> | 1.1 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.03 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 300 | pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 300K |
