1TD6

Crystal structure of the conserved hypothetical protein MP506/MPN330 (gi: 1674200)from Mycoplasma pneumoniae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2004-04-11
Detector	ADSC QUANTUM 210
Wavelength(s)	0.9792
Spacegroup name	I 41 2 2
Unit cell lengths	82.840, 82.840, 225.040
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.400 - 2.500
R-factor	0.244
Rwork	0.244
R-free	0.29800
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.400
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	41.400
High resolution limit [Å]	2.500
Number of reflections	25029
<I/σ(I)>	28.2
Completeness [%]	96.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP (HYDRA CRYSTALLIZATION ROBOT)	8.5	298	0.1 M Tris pH 8.5, 1.5 M Lithium Sulfate monohydrate, VAPOR DIFFUSION, SITTING DROP (HYDRA CRYSTALLIZATION ROBOT), temperature 298K