1TCR

MURINE T-CELL ANTIGEN RECEPTOR 2C CLONE

Experimental procedure

Source type	ROTATING ANODE
Source details	SIEMENS
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1996-06
Detector	MARRESEARCH
Spacegroup name	C 1 2 1
Unit cell lengths	138.000, 72.900, 57.300
Unit cell angles	90.00, 101.20, 90.00

Refinement procedure

Resolution	? - 2.500
R-factor	0.208
Rwork	0.208
R-free	0.28500
Structure solution method	MR/MIR
Starting model (for MR)	ISOLATED BETA CHAIN V(ALPHA) DOMAIN
RMSD bond length	0.011
RMSD bond angle	27.540 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASES
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]		2.600
High resolution limit [Å]	2.500	2.500
Rmerge	0.048	0.237
Number of reflections	18337
<I/σ(I)>	18.3	2.9
Completeness [%]	99.7	99.3
Redundancy	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	6.4 *	22 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	reservoir	ammonium sulfate	1.8 (M)
3	1	reservoir	sodium acetate	50 (mM)
4	1	reservoir	sodium citrate	50 (mM)