1TCF
CRYSTAL STRUCTURE OF CALCIUM-SATURATED RABBIT SKELETAL TROPONIN C
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | SIEMENS |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 32.100, 59.600, 48.000 |
| Unit cell angles | 90.00, 101.10, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.900 |
| R-factor | 0.278 |
| Rwork | 0.278 |
| R-free | 0.35800 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 23.100 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.851) |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.900 |
| Rmerge | 0.087 |
| Number of reflections | 10911 |
| Completeness [%] | 77.5 |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5.6 | 4 * | 40-50% MPD, 50MM SODIUM ACETATE 5MM CACL2, PH 5.6. |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | MPD | 50-60 (%) | |
| 2 | 1 | reservoir | sodium acetate | 50 (mM) | |
| 3 | 1 | reservoir | 5 (mM) |
