1TCA
THE SEQUENCE, CRYSTAL STRUCTURE DETERMINATION AND REFINEMENT OF TWO CRYSTAL FORMS OF LIPASE B FROM CANDIDA ANTARCTICA
Experimental procedure
Spacegroup name | P 21 21 21 |
Unit cell lengths | 62.100, 46.700, 92.100 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 7.500 - 1.550 |
R-factor | 0.157 |
Rwork | 0.157 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.100 |
Phasing software | X-PLOR |
Refinement software | X-PLOR |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 100.000 * |
High resolution limit [Å] | 1.550 * |
Rmerge | 0.040 * |
Total number of observations | 140416 * |
Number of reflections | 37486 * |
Completeness [%] | 95.0 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 3.6 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG4000 | 20 (%) | |
2 | 1 | reservoir | sodium acetate | 50 (mM) | |
3 | 1 | reservoir | isopropanol | 10 (%) | |
4 | 1 | drop | beta-octyl glucoside | 0.6 (%) | |
5 | 1 | drop | protein | 10 (mg/ml) |