1TBX

Crystal structure of SSV1 F-93

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-D
Synchrotron site	APS
Beamline	14-BM-D
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9790, 0.9787, 0.9562
Spacegroup name	P 43 3 2
Unit cell lengths	120.493, 120.493, 120.493
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	14.000 - 2.700
R-factor	0.19448
Rwork	0.191
R-free	0.25945
Structure solution method	MAD
RMSD bond length	0.019
RMSD bond angle	1.924
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.810
High resolution limit [Å]	2.700	2.700
Number of reflections	7936
<I/σ(I)>	41
Completeness [%]	98.9	98.9
Redundancy	9.24

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.6	277	3.25 M NaCl, 120 mM HEPES, pH 7.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K