1TB3
Crystal Structure Analysis of Recombinant Rat Kidney Long-chain Hydroxy Acid Oxidase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1998-04-11 |
| Detector | SBC |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 114.839, 151.100, 222.360 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.300 |
| Rwork | 0.239 |
| R-free | 0.26900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gox |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.050 | 0.286 |
| Number of reflections | 159633 | |
| <I/σ(I)> | 20.6 | 3.4 |
| Completeness [%] | 93.0 | 79.4 |
| Redundancy | 3.8 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | sodium acetate, sodium citrate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
