1TAZ
Catalytic Domain Of Human Phosphodiesterase 1B
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2003-02-15 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 87.232, 87.232, 134.151 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 72.550 - 1.770 |
| R-factor | 0.18228 |
| Rwork | 0.182 |
| R-free | 0.19319 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.084 |
| Data scaling software | ELVES |
| Phasing software | EPMR |
| Refinement software | REFMAC (5.1.25) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.550 | 1.816 |
| High resolution limit [Å] | 1.770 | 1.770 |
| Rmerge | 0.047 | 0.459 |
| Number of reflections | 48382 | |
| <I/σ(I)> | 9.2 | 1.51 |
| Completeness [%] | 98 | |
| Redundancy | 2.5 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | 50% MPD, 100 mM MES, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
