1TAW
BOVINE TRYPSIN COMPLEXED TO APPI
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 277 |
| Detector technology | DIFFRACTOMETER |
| Collection date | 1992-01 |
| Detector | ENRAF-NONIUS FAST |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 94.050, 49.850, 68.800 |
| Unit cell angles | 90.00, 96.31, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.800 |
| R-factor | 0.184 |
| Rwork | 0.184 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ptc |
| RMSD bond length | 0.008 |
| RMSD bond angle | 25.986 * |
| Data reduction software | PROCOR |
| Data scaling software | PROCOR |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.000 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.059 * | 0.080 |
| Number of reflections | 22374 | |
| <I/σ(I)> | 10 | 2 |
| Completeness [%] | 76.6 | 79.3 |
| Redundancy | 3 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.5 | 4 * | used to seeding * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 40 (M) | |
| 2 | 1 | reservoir | PEG3400 | 25 (%(w/v)) | |
| 3 | 1 | reservoir | ammonium acetate | 200 (mM) | |
| 4 | 1 | reservoir | sodium citrate | 100 (mM) |
